Two new methods for recovery and genetic analysis of hybrids after fusion of yeast protoplasts |
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| Authors: | J. F. T. Spencer Dorothy M. Spencer Maria Cristina Schiappacasse H. Heluane Nicola Reynolds Lucía I. de Figueroa |
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| Affiliation: | (1) PROIMI, Avenida Belgrano y Pasaje Caseros, 4000 S.M. de Tucumán, Argentina;(2) Escuela de Ingeniera, Facultad de Ingeniería, Iniversidad Catolica de Valparaiso, Chile;(3) Department of Biological Sciences and Environmental Health, Thames Polytechnic, Wellington Street, SE18 6PF London, UK;(4) CONICET, PROIMI, Argentina |
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| Abstract: | A method for regeneration of yeast protoplasts and fusants in a gelatin-agar mixture, followed by total recovery of the regenerated cells from the gelatin-agar mixture and isolation of the fusants, is described. A one-step method for obtaining intergeneric fusants in which the greater part of the genome is derived fromSaccharomyces cerevisiae, and in which the fusant can be sporulated directly and tetrad analysis carried out without construction of further hybrids, is also described. |
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