Biological in situ characterization of polymeric microbubble contrast agents |
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| Institution: | 1. Centre for BioNano Interactions, School of Chemistry & Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland;2. Surflay Nanotec GmbH, Max-Planck-Strasse 3, 12489 Berlin, Germany;3. Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata. Via della Ricerca Scientifica 00133 Rome, Italy;4. Groningen Research Institute of Pharmacy, Division Pharmacokinetics, Toxicology and Targeting, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands;5. School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom;1. School of Materials Science and Engineering, Changchun University of Science and Technology, Changchun, 130022, PR China;2. Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Changchun, 130103, PR China;3. School of Material and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, PR China;1. Division of Diabetes, Obesity & Metabolism, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR;2. Division of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR;3. Department of Obstetrics & Gynecology, Oregon Health & Science University, Portland, OR;4. Department of Pathology, Oregon Health & Science University, Portland, OR;5. Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR;1. Department of Chemical and Biomolecular Engineering, Faculty of Engineering, National University of Singapore, Singapore 119260, Singapore;2. Department of Pediatrics, National University Health System, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore;1. Flemish Institute for Technological Research (VITO NV), Environmental Risk and Health Unit, Mol, Belgium;2. Institut Català de Nanotecnologia (ICN), Barcelona, Spain;3. Università di Modena e Reggio Emilia, Laboratorio Biomateriali, Modena, Italy;4. Hasselt University, Centre for Environmental Sciences, Diepenbeek, Belgium;1. Laboratory of Advanced Science and Technology for Industry, University of Hyogo, 3-1-2, Koto, Kamigori, Ako, Hyogo 678-1205, Japan;2. Graduate School of Science, University of Hyogo, 3-2-1, Koto, Kamigori, Ako, Hyogo 678-1297, Japan;3. Mazda Motor Corp., 3-1, Shinchi, Fuchu, Aki, Hiroshima 730-8670, Japan |
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| Abstract: | Polymeric microbubbles (MBs) are gas filled particles composed of a thin stabilized polymer shell that have been recently developed as valid contrast agents for the combined use of ultrasonography (US), magnetic resonance imaging (MRI) and single photon emission computer tomography (SPECT) imaging. Due to their buoyancy, the commonly available approaches to study their behaviour in complex media are not easily applicable and their use in modern medicine requires such behaviour to be fully elucidated. Here we have used for the first time flow cytometry as a new high throughput approach that allows characterisation of the MB dispersion, prior to and after exposure in different biological media and we have additionally developed a method that allows characterisation of the strongly bound proteins adsorbed on the MBs, to fully predict their biological behaviour in biological milieu. |
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| Keywords: | Microbubbles Protein corona Flow cytometry Opsonin proteins Dysopsonin proteins Bionano interactions |
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