Cloning and molecular characterization of a novel x-type glutenin gene Glu-D(t)1 from Aegilops tauschii |
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Authors: | Fang Jingye Liu Yong Zhang Change Yang Guangxiao Li Kexiu Shewry Peter R He Guangyuan |
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Institution: | (1) China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Key Laboratory of Molecular Biophysics, Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Luoyu Road 1037, Wuhan, Hubei, 430074, P. R. China;(2) Rothamsted Research (RRes), Harpenden, Hertfordshire, AL5 2JQ, UK |
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Abstract: | A novel gene encoding an x-type high molecular weight glutenin subunit (HMW-GS), designated 1Dx1.1
t
, was isolated from Aegilops tauschii. It is the largest HMW-GS gene reported so far in this species and its product has a slower mobility than that of subunit
1Ax1 in SDS-PAGE. The open reading frame (ORF) of the gene was 2,628 bp, encoding a protein of 874 amino acid residues. Comparisons
of amino acid sequences showed that subunit 1Dx1.1t had high similarity with other 1Dx subunits but also had two unique characteristics. Firstly, a tripeptide of consensus LQE
present in the N-terminal domains of other 1Dx subunits was absent from subunit Dx1.1t. Secondly, three copies of tandem duplications of the tripeptide motif GQQ and a novel tripeptide sequence (GQL) were present
in its central repetitive domain. Phylogenetic analysis showed that subunit 1Dx1.1t clustered with other known 1Dx subunits. |
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Keywords: | Aegilops tauschii HMW-GS Bacterial expression Evolutionary analysis Wheat quality improvement |
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