Complete sequence of ovine alpha s2-casein messenger RNA |
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Authors: | M Boisnard G Petrissant |
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Affiliation: | Laboratoire de Physiologie de la Lactation INRA-CNRZ, 78350 Jouy-en-Josas, France |
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Abstract: | The primary structure of mRNA coding for ovine alpha s2 casein has been determined by chemical sequencing of three cDNA clones and the primer extension products of the longest one. The mRNA was 1,024 nucleotides long, excluding the poly(A) tail. The length of the 5' noncoding, coding and 3' noncoding regions was 53, 669 and 302 nucleotides, respectively. A comparison of the nucleotide sequence of ovine alpha s2-casein and guinea-pig casein A mRNAs revealed an extensive homology in the 5' and 3' noncoding regions. The deduced amino acid sequence of ovine alpha s2-casein was compared with its bovine and guinea-pig counterparts. Moreover, an heterogeneity was evidenced in the mRNA population of the alpha s2-casein. |
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Keywords: | brebis cobaye ADN complémentaire ARN messager séquence nucléotidique ovine guinea-pig cDNA mRNA nucleotide sequence |
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