Epigallocatechin gallate-induced apoptosis does not affect adipocyte conversion of preadipocytes |
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Authors: | Morikawa Keiko Ikeda Chiharu Nonaka Mitsuko Pei Sumi Mochizuki Misa Mori Asuka Yamada Sonoko |
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Affiliation: | Division of Clinical Nutrition, Department of Nutrition, Sagami Women's University, 2-1-1 Bunkyo, Sagamihara, Kanagawa 228-8533, Japan. morikawa_keiko@isc.sagami-wu.ac.jp |
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Abstract: | In the present study, we examined the effect of epigallocatechin gallate (EGCG) on the growth and differentiation of human preadipocyte cells, AML-I. EGCG exhibited cytotoxic activity on AML-I cells, accompanied by the appearance of characteristics of apoptosis by Annexin V-FITC staining method. Among apoptosis-related proteins examined, loss of NF-kappaB and p-Akt, and accumulation of Bad were displayed in EGCG-treated cells by Western blot analysis. Among 6 structure-related catechins including catechin (C), epicatechin (EC), catechin gallate (CG), epigallocatechin (EGC), epicatechin gallate (ECG) and EGCG, the catechins containing galloyl moiety exhibited apoptotic capacity. Interestingly, exposure of AML-I to EGCG increased the amounts of cytoplasmic lipid droplets as well as the expression of fatty acid synthase and peroxisome proliferator activated receptor-gamma proteins. Our results suggest that EGCG induces growth arrest and apoptosis, but does not affect adipocyte conversion of preadipocytes. |
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Keywords: | Apoptosis Catechin Galloyl moiety Preadipocyte Lipogenesis |
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