Treatment of mycoplasma contamination in a large panel of cell cultures |
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Authors: | Hans G Drexler Suzanne M Gignac Zhen-Bo Hu Anne Hopert Elke Fleckenstein Maren Voges Cord C Uphoff |
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Institution: | (1) Department of Human and Animal Cell Cultures, DSM-German Collection of Microorganisms and Cell Cultures, Mascheroder Weg 1B, D-3300 Braunschweig, Germany |
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Abstract: | Summary Mycoplasmal contamination remains a significant impediment to the culture of eukaryotic cells. For certain cultures, attempts
to eliminate the infection are feasible alternatives to the normally recommended disposal of the contaminated culture. Here,
three antibiotic regimens for mycoplasmal decontamination were compared in a large panel of naturally infected cultures: a
1-wk treatment with the fluoroquinolone mycoplasma removal agent (MRA), a 2-wk treatment with the fluoroquinolone ciprofloxacin,
and three rounds of a sequential 1-wk treatment with BM-Cyclin containing tiamulin and minocyclin. These antibiotic treatments
had a high efficiency of permanent cure: MRA 69%, ciprofloxacin 75%, BM-Cyclin 87%. Resistance to mycoplasma eradication was
observed in some cell cultures: BM-Cyclin 0%, MRA 20%, ciprofloxacin 20%. Nearly all resistant contaminants that could be
identified belonged to the speciesMycoplasma arginini andM. orale. Detrimental effects of the antibiotics were seen in the form of culture death caused by cytotoxicity (in 5 to 13% of the
cultures). Alterations of the cellular phenotypic features or selective clonal outgrowth might represent further untoward
side effects of exposure to these antibiotics. Overall, antibiotic decontamination of mycoplasmas is an efficient, inexpensive,
reliable, and simple method: 150/200 (75%) chronically and heavily contaminated cultures were cured and 50/200 (25%) cultures
could not be cleansed and were either lost or remained infected. It is concluded that eukaryotic cell cultures containing
mycoplasmas are amenable to antibiotic treatment and that a cure rate of three-quarters is a reasonable expectation. |
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Keywords: | mycoplasma cell culture antibiotics |
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