| 新疆盐生植物耐盐基因NHX转化甘蓝型油菜及其耐盐性的初步研究 |
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| 引用本文： | 郝东风,兰海燕,陈邦党,张富春.新疆盐生植物耐盐基因NHX转化甘蓝型油菜及其耐盐性的初步研究[J].生物技术通报,2006(3):81-84. |
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| 作者姓名： | 郝东风 兰海燕 陈邦党 张富春 |
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| 作者单位： | 新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,乌鲁木齐,830046 |
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| 基金项目： | 新疆高校创新研究群体基金项目(XJEDU2004G02)，教育部科学技术研究重点项目(205178) |
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| 摘 要： | 以甘蓝型油菜带柄子叶为转化受体,通过根癌农杆菌EHA105(Agrobacterium tumefaciens)介导将盐角草(Salicornia)的Na /H 反向运输体基因NHX导入新疆主栽油菜1khp11品系,获得了抗卡那霉素的再生植株,并对影响遗传转化的一些关键因素进行了研究。实验结果表明:带柄子叶在含有2,4-D的培养基上经过2d短时间的预培养后在菌液浓度OD600为0.3时在28℃摇床浸染15min时卡那抗性绿苗率可达10%￣12%;经过抗性植株的PCR及RT-PCR检测证明外源基因已整合到油菜基因组中,并通过表型实验证实由于外源基因的导入提高了植株的耐盐能力。
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| 关 键 词： | 耐盐基因NHX 新疆盐生植物盐角草 油菜带柄子叶 耐盐能力 |
| 收稿时间： | 2006-02-14 |
| 修稿时间： | 2006年2月14日 |
Introduction of Na+/H+ Transporter Gene NHX From Native Halophyte Salicornia spp in Xinjiang into Brassica Napus and Its salt Tolerance |
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| Hao Dongfeng,Lan Haiyan,Cheng Bangdang,Zhang Fuchun.Introduction of Na+/H+ Transporter Gene NHX From Native Halophyte Salicornia spp in Xinjiang into Brassica Napus and Its salt Tolerance[J].Biotechnology Bulletin,2006(3):81-84. |
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| Authors: | Hao Dongfeng Lan Haiyan Cheng Bangdang Zhang Fuchun |
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| Institution: | College of Life Science and Technology, Xinjiang University, Key Laboratory of Xinfiang Biological Resources and Genetic Engineering, Urumqi 830046 |
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| Abstract: | Cotyledons with petiole of Xinjiang Brassica napus variety 1khp11 variety were transformed with Agrobacterium tumefaciens EHA105,in which is harboring Na /H transport gene NHX of Salicornia,and the kan-resistant were regenerated a native halophyte in xinjiang,and andysed the key factors affecting the genetic transformation.The results showed that kan-resistant plantlets can be obtained and NHX gene has already inserted into genome of oilseed by PCR and RT-PCR.The salt tolerance ability of transgenic oilseed was significant improved by introduction of NHX gene of Salicornia. |
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| Keywords: | Salt tolerant gene NHX Halophyte salicornia in xinjiang Cotyledons with petiole of brassica napus Salt tolerance |
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