Complementation in somatic hybrids indicates four types of nitrate reductase deficient lines in Nicotiana plumbaginifolia |
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Authors: | Lá szló Má rton, Vladimir Sidorov, Grazia Biasini Pá l Maliga |
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Affiliation: | (1) Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary;(2) Present address: Institute of Botany, Ukrainian Academy of Sciences Repina 2, 252601 Kiev, USSR |
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Abstract: | Summary Allelism of nine nitrate reductase deficient (NR–) Nicotiana plumbaginifolia cell lines was tested by complementation after protoplast fusion. Complementation was recognized by the appearance of somatic hybrid colonies growing on a selective NH4+/NO3– medium which cannot support the growth of NR– lines. All five apoenzyme defective (NA) lines were non-complementing and therefore allelic. The apoenzyme and the cofactor defective (NX) lines were complementing, as expected, and gave somatic hybrids with restored nitrate reductase activity. The four cofactor defective lines were found to belong to three complementation groups (NX1 and NX9; NX21; NX24). Two of these (NX21 and NX24) are of new types which have not been previously described in flowering plants. In the somatic hybrids restoration of NR activity was accompanied by the restoration of plant regeneration ability.On leave from: Instituto di Mutagenesi e Differenziamento CNR, Via Svezia, 10, 56100, Pisa, Italy |
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