Flow cytometric evidence for multiple ploidy levels in the endosperm of some gymnosperm species |
| |
Authors: | C. Pichot M. El Maataoui |
| |
Affiliation: | (1) INRA, Unité de Recherches Forestières Méditerranéennes, Equipe de Génétique, Av. Vivaldi, 84000 Avignon, France, FR;(2) Université d’Avignon, Laboratoire de Biologie Végétale Expérimentale, 33 rue L. Pasteur, 84000 Avignon, France, FR |
| |
Abstract: | Considered to be haploid tissue, the endosperm of coniferous trees has been extensively used by forest geneticists. Using laser flow cytometry, we show that endosperm ploidy level depends on the systematic position. The Abies, Cedrus and Pinus species tested exhibited uniform haploid endosperm compared to the diploid DNA content of the corresponding embryo. Endosperm of Cupressaceae contained multiple ploidy levels: Cupressus arizonica, Juniperus oxycedrus and Thuja orientalis endosperms exhibited a mixture of haploid–diploid nuclei, while C. atlantica and C. sempervirens endosperms contained six ploidy levels: 1C, 2C, 3C, 4C, 5C and 6C. Physiological and genetic implications of this original feature are discussed. Received: 17 August 1996 / Accepted: 18 October 1996 |
| |
Keywords: | Gymnosperms Ploidy Endosperm Embryo Flow cytometry |
本文献已被 SpringerLink 等数据库收录! |
|