Formation of GA20 glucosyl conjugates in seedlings ofVicia faba |
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Authors: | P Lattke G Schneider |
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Institution: | 1. Institute of Plant Biochemistry, Academy of Sciences of the German Democratic Republic, Halle (Saale), G.D.R.
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Abstract: | 3H]GA20 (1)1, fed toVicia faba seedlings, was converted to 3H]GA20 glucosyl ester (5) and 3H]GA20-13-0-glucoside (6). The GA20 glucosyl ester (5) was identified by HPLC-RC and by GC-MS of GA20-Me formed by transesterification of (5). The 3H]GA20-Me was crystallized to constant specific radioactivity with authentic GA20-Me. On HPLC-RC the GA20-13-0-glucoside (6) was shown to have the same retention time as an authentic sample. Subsequent enzymic hydrolysis gave a product with an HPLC retention time identical to that of authentic GA20 (1). |
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