Auxin levels in single half nodes ofAvena fatua estimated using high performance liquid chromatography with coulometric detection |
| |
| Authors: | Michael Wright Patrick Doherty |
| |
| Institution: | 1. Developmental Botany, AFRC Weed Research Organization, Begbroke Hill, OX5 1PF, Oxford, UK
2. Department of Biochemistry, University of Bath, BA2 7AY, Claverton Down, Bath, UK
|
| |
| Abstract: | A high performance liquid chromatography (HPLC) based micro-method for estimation of indole-3-acetic acid (IAA) levels in single half nodes from the flowering stalks ofAvena fatua has been developed; this features a dual electrode coulometric electrochemical detector operating at a detection limit of c. 2 pg. Samples were prepared by solvent partitioning and preliminary fractionation with C18 Sep-Pak cartridges. Two stages of reversed phase ion pair HPLC were employed; the first was gradient elution with fluorescent detection, the second, isocratic elution with coulometric detection. The lower limit for estimation of IAA levels in purified extracts was c. 5 pg. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
