Rat brain endoplasmic reticulum calcium pools are anatomically and functionally segregated. |
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| Authors: | A Verma C A Ross D Verma S Supattapone and S H Snyder |
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| Institution: | Johns Hopkins University School of Public Health, Department of Toxicology, Baltimore, Maryland 21205. |
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| Abstract: | Autoradiographic imaging can localize 45Ca2+ selectively accumulated via the Ca2+, Mg2(+)-ATPase into endoplasmic reticulum stores in rat brain slices. 45Ca2+ accumulation is markedly stimulated by oxalate and displays a heterogeneous distribution which resembles the mRNA distribution for a sarcoendoplasmic reticulum Ca2+, Mg2(+)-ATPase. Inositol 1,4,5-triphosphate I(1,4,5)P3] inhibits 45Ca2+ accumulation selectively into regions corresponding to those enriched in I(1,4,5)P3 receptor-binding sites and in Ca2+, -Mg2(+)-ATPase mRNA. Thus rat brain endoplasmic reticulum calcium stores are anatomically and functionally differentiated. |
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