Characterization of DNA sequences associated with residual nuclei of Saccharomyces cerevisiae |
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| Authors: | J A Potashkin J A Huberman |
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| Affiliation: | 1. Faculty of Dental Medicine and Oral Health Sciences, Department of Anesthesia, Faculty of Medicine, Alan Edwards Centre for Research on Pain, McGill University, Montreal, QC, Canada;2. Department of Anesthesiology and Pain Management, Maastricht University Medical Center+, School for Mental Health and Neuroscience (MHeNs), Faculty of Health, Medicine and Life Sciences, University of Maastricht, Maastricht, The Netherlands;3. Department of Pharmacology and Toxicology and Translational Research Initiative for Pain and Neuropathy, Virginia Commonwealth University, Richmond, VA, USA;4. Department of Anesthesiology, Faculty of Health Sciences, Queen''s University, Kingston, ON, Canada;5. Department of Biomedical & Molecular Sciences, Faculty of Health Sciences, Queen''s University, Kingston, ON, Canada;6. Centre for Neuroscience Studies, Queen''s University, Kingston, ON, Canada;7. Department of Microbiology and Immunology, Meakins-Christie Laboratories, Research Institute of the McGill University Health Centre, Montreal, QC, Canada;8. Department of Pain Therapy, Ensemble Hospitalier de la Côte, Morges, Switzerland;9. Pain Therapy Service, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy;10. Department of Anesthesiology and Perioperative Medicine, University of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, PA, USA;11. College of Nursing, University of Iowa, Iowa City, IA, USA;12. Department of Anesthesiology, Centre for Cancer and Organ Diseases, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark;13. Section of Surgical Pathophysiology 7621, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark;14. Department of Anesthesiology, Division of Anesthesiology, Emergency and Intensive Care Medicine, University Medical Center Utrecht, Utrecht, The Netherlands;15. Center for Drug Discovery, Department of Pharmaceutical Sciences, Northeastern University, Boston, MA, USA;16. McGill Interdisciplinary Initiative in Infection and Immunity, McGill Centre for Microbiome Research, Montreal, QC, Canada;17. Department of Psychology, Faculty of Science, Alan Edwards Centre for Research on Pain, McGill University, Montreal, QC, Canada;1. Fondazione IRCCS Istituto Nazionale dei Tumori, Milano, Italy;2. Medica Scientia Innovation Research (MEDSIR), Barcelona, Spain and Ridgewood, NJ, United States;3. International Breast Cancer Center (IBCC), Pangaea Oncology, Quirón Group, Barcelona, Spain;4. Vall d''Hebrón University Hospital, Medical Oncology Department, Spain;5. Vall d''Hebrón Institute of Oncology (VHIO), Barcelona, Spain;6. Oncopole Claudius Regaud-IUCT, CRCT, Inserm, Department of Medical Oncology, Toulouse, France;7. Institut Català d''Oncologia, Breast Cancer Unit and Medical Oncology Department, IDIBELL, L''Hospitalet, Barcelona, Spain;8. Hospital Universitario Virgen del Rocío, Medical Oncology Department, Seville, Spain;9. Fundación Instituto Valenciano de Oncología, Medical Oncology Department, Valencia, Spain;10. Barts ECMC, Barts Cancer Institute, Queen Mary University of London, Barts Hospital NHS Trust, London, United Kingdom;11. University Hospital Mannheim, Germany;12. Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany;13. Institut Universitaire de Cancérologie, AP-HP Sorbonne Université, Paris, France;14. National Center for Tumor Diseases (NCT), Heidelberg University Hospital and German Cancer Research Center, Heidelberg, Germany;15. Hospital del Mar, Medical Oncology, Barcelona, Spain;p. Hospital Universitario La Paz, Medical Oncology Department, Madrid, Spain;q. Royal Cornwall Hospitals NHS Trust, Truro, United Kingdom;r. Medical Oncology Department, Consorcio Hospitalario Provincial de Castellón, Castellón, Spain;s. Hospital Universitari Sant Joan de Reus, Reus, Spain;t. Universidad Europea de Madrid, Faculty of Biomedical and Health Sciences, Department of Medicine, Madrid, Spain;u. Hospital Arnau de Vilanova, Valencia, Spain;v. Universidad Católica de Valencia, Valencia, Spain |
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| Abstract: | We have used two different approaches to determine whether particular DNA sequences are specifically associated with high-salt-treated residual nuclei of Saccharomyces cerevisiae. First, libraries of yeast DNA in phage lambda were probed with nick-translated total nuclear or residual nuclear DNA from unsynchronized yeast cells. None of the plaques gave a significantly stronger or weaker signal with the residual nuclear probe than with the total nuclear probe. Second, DNA was purified from whole nuclei or residual nuclei which had been isolated from cells in G1, G1/S, early S, or nuclear division. This DNA was "dot-blotted" and then probed with specific yeast DNA sequences. Ribosomal DNA was 2- to 3-fold enriched in residual nuclei in late G1, G1/S, and early S, and 2 microns plasmid DNA sequences were 3- to 5-fold depleted during nuclear division and early G1. However, ARS1, TRP1, CEN6, and a telomere sequence were neither enriched nor depleted at any time during the cell cycle. |
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