Chemically phosphorylated protamine: a new substrate for the study of phosphoprotein phosphatase activity. |
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| Authors: | B Ullman R L Perlman |
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| Institution: | Department of Physiology and Program in Cell and Developmental Biology Harvard Medical School Boston, Mass. 02115 USA |
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| Abstract: | A method for the chemical phosphorylation of seryl residues in protamine has been developed. Both non-radioactive and 33P]phosphoprotamine have been prepared and characterized. Phosphoprotein phosphatases from a variety of mammalian tissues release inorganic phosphate from phosphoprotamine. Chemically phosphorylated protamine is a useful new substrate for the study of these phosphoprotein phosphatases. |
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