Polyelectrolyte complex formation mediated immobilization of chitosan-invertase neoglycoconjugate on pectin-coated chitin |
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Authors: | Leissy Gómez Hector L. Ramírez Andrónico Neira-Carrillo Reynaldo Villalonga |
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Affiliation: | (1) Center for Enzyme Technology, University of Matanzas, 44740 Matanzas, Cuba;(2) Faculty of Veterinary and Animal Science, Center for Advanced Interdisciplinary Research in Materials (CIMAT), University of Chile, Santiago, Chile |
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Abstract: | Saccharomyces cerevisiae invertase, chemically modified with chitosan, was immobilized on pectin-coated chitin support via polyelectrolyte complex formation. The yield of immobilized enzyme protein was determined as 85% and the immobilized biocatalyst retained 97% of the initial chitosan-invertase activity. The optimum temperature for invertase was increased by 10 °C and its thermostability was enhanced by about 10 °C after immobilization. The immobilized enzyme was stable against incubation in high ionic strength solutions and was 4-fold more resistant to thermal treatment at 65 °C than the native counterpart. The biocatalyst prepared retained 96 and 95% of the original catalytic activity after ten cycles of reuse and 74 h of continuous operational regime in a packed bed reactor, respectively. |
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Keywords: | Invertase Chitin Enzyme immobilization Enzyme stability Polyelectrolyte complex |
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