Thyroxine-mediated release of diphosphopyridine nucleotide from mitochondrial dehydrogenases |
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| Authors: | EMMELOT P BOS C J |
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| Affiliation: | 1. Department of Biotechnology and Food Technology, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa;2. Institut Universitaire de Technologie, Créteil-Vitry, 61 Avenue du Général de Gaulle, 94010 Créteil cedex, France;3. Agricultural Research Council – Institute for Tropical and Subtropical Crops, Private Bag X11208, Nelspruit 1200, South Africa;4. Department of Chemistry, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa |
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| Abstract: | - 1.1. The inhibitory effect of thyroxine on several DPN-linked dehydrogenases of mouse liver mitochondria has been studied. Since the inhibitions were prevented in the presence of DPN, it was concluded that the hormone released the coenzyme from the enzymatically-active sites.
- 2.2. EDTA, Mg2+, Mn2+ and PVP counteracted or prevented the thyroxine-mediated release of DPN from the mitochondrial glutamate dehydrogenase.
- 3.3. The results lend additional support to the view that thyroxine labilizes the functional integrity of the mitochondria rather than acting upon phosphorylation in a specific way.
- 4.4. Diethylstilboestrol was found to inhibit both the mitochondrial and crystalline glutamate dehydrogenase. The inhibition of the mitochondrial enzyme could be overcome or counteracted by adding DPN, EDTA, Mn2+ or Mg2+, but not by PVP.
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