Changes of Uric Acid Level in Rat Brain After Focal Ischemia |
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| Authors: | Hideaki Kanemitsu Akira Tamura Keiji Sano Takeo Iwamoto Masahiko Yoshiura Keiji Iriyama |
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| Affiliation: | Department of Neurosurgery, Teikyo University School of Medicine, Tokyo, Japan;Division of Biochemistry, Central Research Laboratory, The Jikei University School of Medicine, Tokyo, Japan |
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| Abstract: | Changes of uric acid level in rat cerebral hemisphere after left middle cerebral artery (MCA) occlusion were studied by reversed-phase HPLC with electrochemical detection. Uric acid level in the normal group was 2.98 nmol/g tissue. Uric acid concentration of the left hemisphere in the left MCA-occluded group progressively increased after occlusion, and reached a maximum value of 67.26 nmol/g tissue 24 h after ischemia. Uric acid levels in the right hemisphere remained unchanged. Uric acid concentration of the left hemisphere in sham-operated group was 9.29 nmol/g tissue 24 h after the operation. |
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| Keywords: | Uric acid Rat brain Ischemia Liquid chromatography Electrochemical detection |
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