Creating new genes by plasmid recombination in Escherichia coli and Bacillus subtilis |
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Authors: | Gomez Ana Galic Tatjana Mariet Jean-François Matic Ivan Radman Miroslav Petit Marie-Agnès |
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Institution: | Eucodis GmbH, Wien, Austria. |
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Abstract: | Gene shuffling is a way of creating proteins with interesting new characteristics, starting from diverged sequences. We tested an alternative to gene shuffling based on plasmid recombination and found that Bacillus subtilis efficiently recombines sequences with 4% divergence, and Escherichia coli mutS is more appropriate for sequences with 22% divergence. |
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