Thimerosal stimulates focal adhesion kinase and cytoskeletal changes by redox modulation |
| |
Authors: | Kim Euikyung Kim Jung Hwan Kim Hyeon Soo Ryu Sung Ho Suh Pann-Ghill |
| |
Institution: | POSTECH Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, San 31, Hyoja-Dong Nam-Gu, Pohang, Kyungbuk 790-784, South Korea. |
| |
Abstract: | Thimerosal is one of the most widely used preservatives and has been reported to cause chemically mediated side effects. However, the mechanism of the side effects is not clearly understood yet. In the present study, we showed that HeLa S cells treated by thimerosal generated reactive oxygen species (ROS). Thimerosal-generated ROS stimulated the tyrosine phosphorylation of focal adhesion kinase (FAK) and also induced cytoskeletal changes. Pretreatment with intracellular calcium chelator, BAPTA did not block the thimerosal-mediated FAK tyrosine phosphorylation. On the other hand, either FAK inhibitor, tyrphostin or ROS scavenger, N-acetyl-L-cysteine (NAC) suppressed the tyrosine phosphorylation and cytoskeletal changes. These results suggest that thimerosal seems to induce FAK tyrosine phosphorylation and cytoskeletal changes by ROS generation but not by intracellular calcium mobilization. We think the present finding can be an important clue to understanding the mechanism of thimerosal-mediated side effects, such as contact dermatitis, and allergy. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|