脊髓TrkC mRNA的表达及其坐骨神经损伤后的变化

目的和方法：本文利用原位杂交和点杂交的方法，对ＴｒｋＣ ｍＲＮＡ大鼠脊髓组织中的分布与坐骨神经损伤后的表达变化进行了研究。结果：ＴｒｋＣ ｍＲＮＡ几乎存在于所有脊髓神经元与胶质细胞中，在坐骨神经损伤后１ｄ表达增加，以后降到较低水平，到１４ｄ又再次升高，但第二峰较第一峰为低。

EXPRESSION OF TrkC mRNA IN RAT SPINAL CORDS AND ITS CHANGES FOLLOWING CRUSHING SCIATIC NERVES

Aim and Methods: NT3 is an important member of NGF family and performs functions through TrkC and p75 receptors. In situ hybridization and dot blotting were used to study distribution and cellular localization of TrkC mRNA in rat spinal cords and its changes following crushing sciatic nerve.Results: The expression of TrkC mRNA in nearly all neurons suggested widely neurotrophic functions of NT3 for the cells. Dot blotting shows TrkC mRNA increased 1 day after axotomy, went down 3 days and remained at low level up to 10 days, rose again at 14 days, but second peak was lower than first peak. The upregulation of TrkC mRNA 1 day after axotomy may be resulted as stress factor, the second upregulation 14 days associated with rearrangement of regenerating nerve into target tissues.Conclusion: The changes of TrkC mRNA may be due to conversion of metabolic status of axotomized of neurons from transmitting mode to growth mode.