| 利用染色体C-分带和双色荧光原位交杂技术鉴定普通小麦-黑麦-簇毛麦双重易位系1RS·1BL，6VS·6AL |
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| 引用本文： | 亓增军,刘大钧,陈佩度.利用染色体C-分带和双色荧光原位交杂技术鉴定普通小麦-黑麦-簇毛麦双重易位系1RS·1BL，6VS·6AL[J].遗传学报,2001,28(3):267-273. |
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| 作者姓名： | 亓增军 刘大钧 陈佩度 |
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| 作者单位： | 南京农业大学农业部作物细胞遗传重点开放实验室，南京 210095 |
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| 基金项目： | 国家高技术研究发展计划(863)(Z-17-04-01)和国家自然科学基金重大项目(39893350)资助 |
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| 摘 要： | 为改进小麦-黑麦1RS·1BL易位系的抗病性，丰富栽培小麦品种的遗传多样性，本文通过1RS·1BL与6VS·6AL易位系杂交，在后代中利用染色体C-分带技术从杂种F
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| 关 键 词： | 普通小麦柡诼髺簇毛麦双重易位系 1BL· 1RS，6VS· 6AL C-分带 双色荧光原位杂交 |
| 文章编号： | 0379-4172(2001)03-0267-07 |
| 修稿时间： | 2000年9月8日 |
Development and
Identification of T. Aestivum-S. Cereale-H. Villosa Double Translocation Line 1RS·1BL,
6VS·6AL via Chromosome C-banding and Dual Color FISH |
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| Abstract: | In order to enhance the disease resistance of wheat-rye 1BL·1RStranslocation line and broaden its genetic bases, a 1RS·1BL line was crossed to 6VS·6AL and from its F2 progenies, a double translocation line designated as 1RS·1BL, 6VS·6AL (2n＝42) was identified by (root tip cell) RTC chromosome C-banding. Its mean chromosome configuration at PMC MI was 19.14＋1.86 indicating genetic stability. Rye and H. Villosa genomic DNA labelled by biotin-and digoxingenin-11-Dutp respectively were used as probes simultaneously for dual color FISH identification. The results confirmed the C-banding results. Rye and H. Villosa chromatin after FISH expressed green or red signals respectively in both RTC and PMC of the double translocation line. This line showed normal fertility, desirable agronomic traits and resistance to powdery mildew, and might be of interest for wheat improvement. |
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