H-2 control of expression of an idiotype shared by normal B cells and a B-cell lymphoma |
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Authors: | Gail Abendroth Bishop Geoffrey Haughton |
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Affiliation: | (1) Department of Microbiology and Immunology and the Lineberger Cancer Research Center, The University of North Carolina, 27514 Chapel Hill, North Carolina |
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Abstract: | The spleens of normal B10,H-2aH-44bp/Wts (2a4b) mice; contain cells which, in response to mitogen stimulation, secrete hemolytic antibody specific for a determinant present on both sheep and bromelain-treated mouse erythrocytes. These cells were found to be Ly-1 positive. Approximately 50% of these cells bear surface immunoglobulin (sIg) with the same idiotype as the sIg of a 2a4b-derived B-cell lymphoma, CH12. Backcross analysis revealed H-2 control of the frequency of the idiotype-positive B cell. The regulatory gene did not correlate with the Igh-1 allotype, and analysis of 22 inbred mouse strains mapped the gene to the I-E subregion. Surprisingly, only strains homozygous for Ekexpressed the idiotype, and expression was a recessive trait. Possible mechanisms for this control of idiotype expression and its relation to lymphomagenesis are discussed.Abbreviations used in this paper 2a4b B10.H-2aH-4bp/Wts - Br-MRBC bromelain-treated mouse erythrocytes - C complement - LPS lipopolysaccharide W - pfc plaque-forming cells - sIg surface immunoglobulin - SRBC sheep erythrocytes - Ts T suppressor. |
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