Characterization of a Bacillus subtilis 64-kDa DNA polymerase X potentially involved in DNA repair |
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Authors: | Baños Benito Lázaro José M Villar Laurentino Salas Margarita de Vega Miguel |
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Institution: | Instituto de Biología Molecular “Eladio Viñuela” (CSIC), Centro de Biología Molecular “Severo Ochoa” (CSIC-UAM), C/Nicolás Cabrera 1, Cantoblanco, 28049 Madrid, Spain |
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Abstract: | Bacillus subtilis gene yshC encodes a 64-kDa family X DNA polymerase (PolXBs), which contains all the critical residues involved in DNA and nucleotide binding as well as those responsible for catalysis of DNA polymerization, conserved in most family X members. Biochemical analyses of the purified enzyme indicate that PolXBs is a monomeric and strictly template-directed DNA polymerase, preferentially acting on DNA structures containing gaps from one to a few nucleotides and bearing a phosphate group at the 5' end of the downstream DNA. The fact that PolXBs is able to conduct filling of a single-nucleotide gap, allowing further sealing of the resulting nick by a DNA ligase, points to a putative role in base excision repair during the B. subtilis life cycle. |
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Keywords: | BSA bovine serum albumin TdT terminal deoxynucleotidyl transferase PolX family X of DNA polymerases BER base excision repair HhH helix-hairpin-helix dRP 5&prime -deoxyribose 5&prime -phosphate ORF open reading frame EDTA ethylenediaminetetraacetic acid dNMP deoxyribonucleoside 5&prime -monophosphate ddNTP 2&prime -3&prime -dideoxynucleoside 5&prime -triphosphate |
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