Analysis of the germination of spores of Bacillus subtilis with temperature sensitive spo mutations in the spoVA operon |
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Authors: | Vepachedu Venkata Ramana Setlow Peter |
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Institution: | Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06032, USA |
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Abstract: | A Bacillus subtilis strain with a base substitution in the ribosome-binding site of spoVAC was temperature sensitive (ts) in sporulation and spores prepared at the permissive temperature were ts in L-alanine-triggered germination, but not in germination with Ca2+-dipicolinic acid (DPA) or dodecylamine. Spores of a ts spo mutant with a missense mutation in the spoVAC coding region were not ts for germination with l-alanine, dodecylamine or Ca2+-DPA. These findings are discussed in light of the proposal that SpoVA proteins are involved not only in DPA uptake during sporulation, but also in DPA release during nutrient-mediated spore germination. |
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Keywords: | Sporulation Spore germination SpoVA proteins Dipicolinic acid |
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