Functional and molecular analysis of mitochondria in thyroid oncocytoma |
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Authors: | Doris Ebner Gerhard Rodel Iris Pavenstaedt Otto Haferkamp |
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Institution: | 1. Institut für Pathologie der Universit?t Ulm, Albert-Einstein-Allee 11, W-7900, Ulm, Federal Republic of Germany
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Abstract: | We report a functional and molecular analysis of nine oncocytic tumors of the human thyroid. In all the abundance of mitochondria
observed ultrastructurally was accompanied by an increase in enzymatic activities of respiratory complexes I (NADH dehydrogenase),
II (succinate dehydrogenase) IV (cytochrome c oxidase), and V (ATPase). Western blot analysis failed to detect uncoupling
protein in the tumors. The elevated respiratory enzyme activities were paralleled by an increase in the mitochondrial DNA
content. Restriction analysis of mitochondrial DNA gave no indication of heteroplasmy or other gross alterations. We conclude
that the mitochondrial proliferation in oncocytic tumors is probably not the result of a compensatory mechanism for the deficiency
in enzyme complexes of the mitochondrial respiratory chain. |
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Keywords: | Mitochondria Mitochondrial DNA Respiratory enzymes Uncoupling protein Thyroid onco-cytic tumor |
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