Synthesis of glyceryl ferulate by immobilized ferulic acid esterase |
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Authors: | Takemasa Matsuo Takashi Kobayashi Yukitaka Kimura Moriyasu Tsuchiyama Tadanobu Oh Tatsuji Sakamoto Shuji Adachi |
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Affiliation: | (1) Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku Kyoto, 606-8502, Japan;(2) Research and Development Department, Okumoto Flour Milling Co., Ltd., Kaizuka Osaka, 597-8515, Japan;(3) Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai Osaka, 599-8531, Japan |
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Abstract: | Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL “Amano” from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50°C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25°C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues. |
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Keywords: | Ferulic acid Ferulic acid esterase Glyceryl ferulate Immobilized enzyme Repeated batch reaction |
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