Primary structure of the asparagine-563-linked carbohydrate chain of an immunoglobulin M from a patient with Waldenstrom's macroglobulinemia. A reinvestigation by 500-MHz H-NMR spectroscopy |
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Authors: | H Van Halbeek L Dorland J F Vliegenthart |
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Affiliation: | 1. Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261 USA;2. Division of Gastroenterology, University of Texas, Health Science Center at Houston Medical School, Houston, TX 77025 USA |
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Abstract: | The activity of microsomal cholesterol 7α-hydroxylase is shown to be increased in vitro by ATP, Mg2+, and a cytosolic protein fraction. There was a loss of enzyme activity in the presence of E. coli alkaline phosphatase which was proportional to the amount of phosphatase. Much of this loss was recovered upon addition of ATP, Mg2+, and a cytosolic protein fraction. |
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