Biofilm formation by <Emphasis Type="Italic">Candida albicans</Emphasis> isolated from intrauterine devices |
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Authors: | Priyanka Lal Vishnu Agarwal Parul Pruthi Ben M J Pereira M R Kural Vikas Pruthi |
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Institution: | (1) Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247 667, India;(2) High Altitude Plant Physiology Research Centre, HNB Garhwal University, Post Box - 14, Srinagar, Garhwal, Uttaranchal, India;(3) Institute Hospital, Indian Institute of Technology Roorkee, Roorkee, 247 667, India |
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Abstract: | Our survey revealed that infected intrauterine devices (IUDs) recovered from patients suffering from reproductive tract infections
(RTIs) were tainted with Candida biofilm composed of a single or multiple species. Scanning electron microscopy (SEM) analysis of C. albicans biofilm topography showed that it consists of a dense network of mono- or multilayer of cells embedded within the matrix
of extracellular polymeric substances (EPS). Confocal scanning laser microscopy (CSLM) and atomic force microscopy (AFM) images
depicted that C. albicans biofilms have a highly heterogeneous architecture composed of cellular and noncellular elements with EPS distributed in the
cell-surface periphery or at cell-cell interface. Biochemical analysis showed that EPS produced by C. albicans biofilm contained significantly reduced total carbohydrate (40%), protein (5%) and enhanced amount of hexosamine (4%) in
contrast to its planktonic counterparts. The in vitro activity of antifungal agents amphotericin B, nystatin, fluconazole and chlorhexidine against pre-formed C. albicans biofilm, assessed using XTT (2,3-bis2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) reduction assay revealed
increased resistance of these infectious biofilm (50% reduction in metabolic activity at a concentration of 8, 16, 64, 128
μg/ml respectively) in comparison to its planktonic form. |
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Keywords: | Biofilm Candida albicans EPS XTT |
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