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Transformation of protoplasts ofCellulomonas flavigena
Authors:Bernardo Pulido-Vega  Teresa Ponce Noyola  Amelia Farrés  Jaime Alvarez de la Cuadra
Institution:(1) Department of Biotechnology and Bioengineering, CINVESTAV-IPN, C.P. 07000, México, D.F., México;(2) Institute of Biomedical Research, UNAM Ciudad Universitaria, México, D.F.;(3) Biotechnology, CIATEJ, Guadalajara, Jal., México
Abstract:Summary Protoplasts ofCellulomonas flavigena (Cms) were transformed with plasmid pC194. Transformation frequency was 2.72×10–3 in MR-1 regeneration medium with 2 mgrg/ml chloramphenicol. Transformation conditions are described.
Keywords:Cellulomonas flavigena  Protoplast  Transformation
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