Regulatory effect of diosgenin on lipogenic genes expression in high-fat diet-induced obesity in mice |
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Authors: | Sahar Khateeb Aishah Albalawi Adel Alkhedaide |
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Affiliation: | aBiochemistry Division, Department of Chemistry, Faculty of Science, Fayum University, Fayum, Egypt;bBiochemistry Department, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia;cBiology Department, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia;dDepartment of Medical Laboratory, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia |
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Abstract: | Obesity is one of the most serious health problems in the world, increasing the risk of other chronic diseases. Alterations in fatty acid synthesis related genes are crucially involved in obesity progression. Diosgenin (DG) was one of the phytosterols compounds with vital activity against lipid disorders. Therefore, this study was intended to evaluate the protective effect of DG on lipogenesis in the high-fat diet (HFD)-induced obesity in mice, via investigating the expression of two of the fatty acid synthesis–involved genes; sterol regulatory element-binding protein (SREBP-1c) and fatty acid synthase (FASN) genes. Thirty adult male mice were divided into 3 groups. Control group, fed with normal diet; HFD group, mice fed with a high-fat diet and HFD + DG group, mice fed with a high-fat diet and supplemented in parallel with DG for 6 consecutive weeks. The effect of DG on Body weights, liver enzymes, lipid profile, were evaluated. Histopathological fatty changes as well as SREBP-1c and FASN gene expression were also investigated. DG significantly alleviated body weight gain, adjusted liver enzymes, and improved lipid profile. Additionally, DG ameliorated the histopathological changes by reducing the lipid vacuoles and hence the hepatosteatosis. Accordingly, DG significantly downregulated the two-fold increase in the SREBP-1c and FASN gene expression observed in the HFD group. In conclusion, DG possesses a beneficial impact against diet-induced obesity in mice, which makes it a good candidate for NAFLD and obesity prevention. |
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Keywords: | Diosgenin FASN SREBP-1c Non-alcoholic fatty liver disease Obesity |
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