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A fluorometric assay for biotinidase |
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| Authors: | H Ebrahim K Dakshinamurti |
| Abstract: | An assay for biotinidase using biocytin, the natural substrate, is described. The fluorometric procedure uses 1,2-diacetylbenzene which reacts selectively with lysine allowing its direct determination in mixture with biocytin. We have examined the applicability of the assay using human serum biotinidase. |
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