Intracellular targeting of calmodulin mRNAs in primary hippocampal cells. |
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Authors: | Elod Kortvely Szilvia Varszegi Arpad Palfi Karoly Gulya |
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Affiliation: | Department of Zoology and Cell Biology, University of Szeged, Szeged, Hungary. |
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Abstract: | We investigated the intracellular distribution of the mRNAs corresponding to the three non-allelic CaM genes in cultured hippocampal cells by in situ hybridization with digoxigenin-labeled gene-specific riboprobes. In neurons the perikaryon was heavily stained and strong dendritic mRNA targeting was detected for all three CaM genes. The color labeling exhibited a punctate distribution, suggesting that CaM mRNAs are transported in RNA granules. Immunocytochemistry for S100 demonstrated that glial cells express CaM mRNAs at a very low level. A minority of the cultured cells were negative for either labeling. |
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