Inactivation of SV40 replication by derivatives of benzo[a]pyrene. |
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Authors: | G T Chang R G Harvey W T Hsu S B Weiss |
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Affiliation: | 2. The Department of Biochemistry The University of Chicago Chicago, Illinois 60637 USA;1. Microbiology The University of Chicago Chicago, Illinois 60637 USA;11. The Ben May Laboratory for Cancer Research The University of Chicago Chicago, Illinois 60637 USA |
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Abstract: | When African green monkey kidney cell lines, infected with simian virus 40, were exposed to benzoa]pyrene-7,8-dihydrodiol or -benzoa]pyrene-7,8-dihydrodiol-9,10-epoxide, inhibition of progeny virus formation was observed. Alkylation of SV40 DNA with -BPDE inhibits the infectivity of this viral DNA; however, the inactivation does not follow a single-hit mechanism. Studies on 3H]thymidine incorporation indicate that SV40 DNA synthesis is markedly impaired for the first 12 hours following BPDE treatment; 24 to 36 hours later, however, SV40 DNA synthesis is almost normal. These data suggest that the inhibition of SV40 DNA synthesis by BP derivatives is reversible and that the observed reduction in viral titer requires some other explanation. |
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