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A radioimmunoassay for plasma corticosterone |
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| Authors: | H. A. Gross H. J. Ruder K. S. Brown M. B. Lipsett |
| Affiliation: | a Human Genetics Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014, USA b National Insitute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20014, USA |
| Abstract: | A radioimmunoassay for plasma corticosterone has been developed. Antiserum against corticosterone was produced in rabbits immunized with corticosterone -21- hemisuccinate conjugated to bovine serum albumin. The assay was practical and reliable. The coefficient of variation between assays was ± 23% and among assays was ± 8%. Plasma corticosterone of mice is measured readily by assaying directly aliquots of a methylene chloride extract of 40 μl of plasma. The mean plasma corticosterone concentration of mice was similar to that obtained by other methods. |
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