Modeling of ethanol fermentation using Zymomonas mobilis ATCC 10988 grown on the media containing glucose and fructose |
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Affiliation: | 1. Department of Chemical Engineering, National Chung Cheng University, Chiayi, 621, Taiwan;2. Department of Chemical Engineering, Chung Yuan Christian University, Chung-Li, 320, Taiwan;1. College of Life Sciences, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, China;2. Biomass Energy Center for Arid and Semi-arid Lands, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, China;3. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, China;4. College of Environment Science and Engineering, Shaanxi University of Science & Technology, Xi’an 710021, Shaanxi, China;3. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;4. College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin 300457, China;5. Laboratory of Marine Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan;6. Laboratory of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, 2-8-30 Kounodai, Ichikawa-shi, Chiba 272-0827, Japan;1. Interfaculty Institute of Biochemistry, University of Tuebingen, Hoppe-Seyler-Str. 4, D-72076 Tuebingen, Germany;2. Laboratory of Mass Spectrometry, Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Strasse 10, D-13125 Berlin, Germany;1. The Joint Graduate School of Energy and Environment (JGSEE),King Mongkut''s University of Technology Thonburi, Bangkok, Thailand;2. National Center for Genetic Engineering and Biotechnology (BIOTEC),National Science and Technology Development Agency, Pathum Thani, Thailand;3. National Nanotechnology Center (NANOTEC),National Science and Technology Development Agency, Pathum Thani, Thailand |
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Abstract: | A model of ethanol fermentation by Zymomonas mobilis ATCC 10988 on the medium containing glucose and fructose is proposed. This model was developed on the basis of metabolic analysis and many experimental findings. When glucose was used as the substrate, the dependence of the carbon fraction (α) assimilating to biomass on the specific growth rate (μ) could be well correlated to α = 0.25μ + 0.012. This correlation resulted in a novel equation for specific glucose uptake rate, which could describe the Z. mobilis fermentation in both batch and continuous modes. When fructose and glucose were both presented in the liquid medium, the model could predict the uptake of glucose and fructose as well as the formation of biomass, ethanol and sorbitol by Z. mobilis. All parameters used in the model were independently evaluated on the basis of various experimental findings. Good agreement was found between the model predictions and data of Z. mobilis fermentation on media containing both glucose and fructose. The proposed model could also describe the behavior of ethanol fermentation on sucrose medium supplemented with immobilized invertase. |
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