High-performance liquid chromatographic determination of 1,3-diethyl-2-thiobarbituric acid–malonaldehyde adduct in fish meat

The reaction conditions of 1,3-diethyl-2-thiobarbituric acid (DETBA)–malonaldehyde (MA) adduct formation were examined in order to analyze MA in fish tissue by high-performance liquid chromatography. A reaction mixture containing 4 mM butyl hydroxytoluene was heated at 100°C for 150 min and the DETBA–MA adduct formed was separated by a Inertsil ODS column for 20 min. The detection limit was 5 pmol.