Purification of xylanase from Trichoderma viride by precipitation with an anionic polymer Eudragit S 100 |
| |
| Authors: | M N Gupta Dong Guoqiang Rajni Kaul Bo Mattiasson |
| |
| Institution: | (1) Department of Chemistry, Indian Institute of Technology, 110016 Hauz Khas, New Delhi, India;(2) Department of Biotechnology, Chemical Center, Lund University, S-221 00 Lund, Sweden |
| |
| Abstract: | Summary Endo-xylanase from T. viride has been purified 4.2 fold by precipitation with a commercially available enteric polymer Eudragit S-100. Electrophoretic analysis also indicated removal of contaminant proteins. The enzyme could be recovered in more than 89% yield. The binding of the enzyme to the polymer was predominantly by electrostatic interaction. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
