Evaluation of some garlic (Allium sativum L.) mutants resistant to white rot disease by RAPD analysis |
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| Authors: | I NABULSI B AL-SAFADI N MIR ALI M I E ARABI |
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| Institution: | Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, PO Box 6091, Damascus, Syria |
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| Abstract: | Random amplified polymorphic DNA (RAPD) analysis was used to evaluate genetic diversity among eight garlic mutants resistant to white rot disease (Sclerotium cepivorum). Twelve of the 13 synthetic random primers were found to identify polymorphism in amplification products. Mutants characterised with moderate resistance to white rot were closely related to the control using cluster and correlation analyses. On the other hand, highly resistant mutants were quite distant from the control with low correlation coefficients. The banding patterns produced by primer OPB‐15 (GGAGGGTGTT) with highly resistant mutants may by used as genetic markers for early selection of resistant plants. |
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| Keywords: | Garlic mutant RAPD white rot |
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