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| C57BL/6J-HBV转基因小鼠的繁育与检测 | |
| 引用本文： | 田枫,康爱君,董宇红,郭长占,郑杰,王美华,周淑佩,王兆绰,席宏丽,曾争. C57BL/6J-HBV转基因小鼠的繁育与检测[J]. 中国实验动物学报, 2002, 10(1): 61-63 |
| 作者姓名： | 田枫 康爱君 董宇红 郭长占 郑杰 王美华 周淑佩 王兆绰 席宏丽 曾争 |
| 作者单位： | 1. 北京大学医学部实验动物科学部,北京,100083 2. 合作单位,北京大学第一医院传染科,100034 3. 北京大学基础医学院免疫学系,100083 4. 北京大学基础医学院病理学系,100083 |
| 基金项目： | 北京市科委专项资金资助项目 ( 95 498170 0 ) |
| 摘 要： | 目的 摸清C57BL 6J -HBV转基因小鼠的繁育规律 ,建立可靠的HBsAg表达检测方法。方法 对C57BL 6J-HBV转基因小鼠两种不同交配方式的繁殖性能和HBsAg的表达情况进行比较研究 ;应用免疫组化的方法证实血清学诊断HBsAg的准确性并用激光扫描共聚焦显微镜确定HBsAg在肝细胞中的表达部位。结果与结论 从繁殖性能来看 ,两种交配方式窝产仔数差异不显著 (P >0 .0 5 ) ,而离乳数差异具有显著性 ( 0 .0 1 |
| 关 键 词： | 转基因小鼠 肝炎表面抗原 乙型 繁殖 |
| 文章编号： | 1005-4847(2001)04-0061-03 |
| 修稿时间： | 2001-03-19 |
Breeding and Examination of C57BL/6J-HBV Transgenic Mice |
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| TIAN Feng,KANG Aijun,DONG Yuhong,GUO Changzhan,ZHENG Jie,WANG Meihua,ZHOU Shupei,WANG Zhaochuo,XI Hongli,ZENG Zheng. Breeding and Examination of C57BL/6J-HBV Transgenic Mice[J]. Acta Laboratorium Animalis Scientia Sinica, 2002, 10(1): 61-63 | |
| Authors: | TIAN Feng KANG Aijun DONG Yuhong GUO Changzhan ZHENG Jie WANG Meihua ZHOU Shupei WANG Zhaochuo XI Hongli ZENG Zheng |
| Abstract: | Objective To find out the breeding rule of C 57 BL/6J-HBV transgenic mice and to establish a credible examination method of HBsAg. Methods Breeding characterization and expression of HBsAg of C 57 BL/6J-HBV transgenic mice in two different matings were compared. The nicety of serum diagnosis of HBsAg was conformed by immunohistochemistry, and the localization of expressed products in the liver cells was determined by laser scanning confocal microscopy.Results and Conclnsion The litter number of two different mating methods is nonsignficant( P >0.05),however the weaning number is significant(0.01< P <0.05).Positive rate of intercross mice is higher than that of backcross mice. Laser scanning confocal microscopy determines that HBsAg is expressed in the cell membrane and cytoplasm. |
| Keywords: | HBV-transgenic mice Hepatitis B surface antigens Breeding |
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