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分子生物光子学的进展
引用本文:王桂英,付国,徐至展. 分子生物光子学的进展[J]. 生命科学, 2008, 20(1): 14-21
作者姓名:王桂英  付国  徐至展
作者单位:中国科学院上海光学精密机械研究所,强场激光物理国家重点实验室,上海201800
基金项目:国家重点基础研究发展计划(973计划) , 国家自然科学基金委员会仪器专项 , 国家自然科学基金
摘    要:随着分子生物学和标记技术的进步,光学成像和分析有了很快的发展,无论是从理论上还是应用上都有所突破,展现了良好的前景。本文综述了近年来在分子生物探测中光子学的情况,简介了几种突破光学衍射极限的探测方法,着重阐明了与生化技术密切相关的荧光共振能量转移和荧光相关光谱显微术及其在分子生物学中的应用。

关 键 词:光学成像和分析  生物光子学  单分子探测
文章编号:1004-0374(2008)01-0014-08
收稿时间:2007-10-29
修稿时间:2007-12-09

An advance in molecular biophotonics
WANG Gui-ying,FU Guo,XU Zhi-zhan. An advance in molecular biophotonics[J]. Chinese Bulletin of Life Sciences, 2008, 20(1): 14-21
Authors:WANG Gui-ying  FU Guo  XU Zhi-zhan
Affiliation:(State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China)
Abstract:Whether on theory or in applications, optical imaging and analysis have gained great progresses with the developments of biological studies and labeling techniques and which exhibits a good prospect. Recently, the biophotonics advances and several new ways of breaking diffraction limit on the optical imaging are described in this paper. In particular, the progresses on the fluorescence resonance energy transfer microscopy and fluorescence correlation spectroscopy, as well as their applications for resolving molecular biologic problem are discussed in detail.
Keywords:optical imaging and analysis   biophotonics   single molecule detection
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