Association of polyamines in governing the chilling sensitivity of maize genotypes |
| |
Authors: | Canhong Gao Jin Hu Sheng Zhang Yunye Zheng Allen Knapp |
| |
Institution: | (1) Department of Agronomy, Zhejiang University, Hangzhou, 310029, China;(2) Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA |
| |
Abstract: | Chilling stress is an important constraint of global production of maize. This study was undertaken to compare the chilling
responses of different maize seedling tissues and to analyze changes in polyamines as a result of chilling stress. Reponses
to chilling were characterized in two maize (Zea mays L.) inbred lines, ‘HuangC’ and ‘Mo17’, that putatively differ in chilling sensitivity. Seedlings were exposed to low temperature
(5°C) and chilling injury was estimated by electrical conductivity (EC), malonaldehyde (MDA) concentration, and by changes
in putrescine (Put), spermidine (Spd) and spermine (Spm) concentrations in root, mesocotyl, and coleoptile tissues. Membrane
permeability (as measured by EC), MDA concentrations and Put concentrations in the three tissue of maize seedlings increased
after chilling stress, except for the Put concentration in roots. Spd and Spm concentrations in the three tissues of seedlings
decreased after chilling stress. The EC for cold stressed tissues were lower in HuangC than Mo17. Also, the EC of coleoptile
tissues were lower than for mesocotyl in both inbred lines. We suggest that mesocotyl tissue can be used to evaluate cold
tolerance in maize. Stepwise regression analyses showed that chilling injury in roots was generally correlated with Spd concentration
while in the mesocotyl injury was mainly correlated with Put and Spd concentrations. Spermidine and Spm concentrations in
the coleoptile were correlated with chilling injury. Characteristics changes of polyamines in chill-tolerant maize seedling
combined with regression analysis are a reliable method for evaluating chill tolerance in maize lines. |
| |
Keywords: | Maize Inbred lines Polyamines Chilling injury parameters Stepwise regression analysis |
本文献已被 SpringerLink 等数据库收录! |
|