Oral vaccination of mice with Tremella fuciformis yeast-like conidium cells expressing HBsAg |
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Authors: | Dong-Il Shin Kyu-Seon Song Hee-Sung Park |
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Affiliation: | 1.Department of Biotechnology,Catholic University of Daegu,Gyeongsan,Korea |
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Abstract: | Tremella fuciformis yeast-like conidium (YLC) cells were transformed by co-cultivation with Agrobacterium cells harboring the hepatitis B surface antigen (HBsAg) gene construct under the control of the CaMV35S promoter. Integration of HBsAg DNA into the YLC genome was confirmed by PCR and dot-blot hybridization. Immunoblotting verified expression of the recombinant protein. Oral administration of YLC cells expressing HBsAg in mice significantly increased anti-HBsAg antibody titer levels using a double prime-boost strategy that combined parenteral and oral HBsAg boosters. |
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