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The nature of a covalent bond between the relaxation protein and ColE1-DNA in the relaxation complex |
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| Authors: | K L Zhuklis Iu F Drygin A A Bogdanov |
| Abstract: | O-[32P]phosphoserine was found to be the only phosphoamino acid in the acid hydrolysate of the [32P]ColE1 DNA-peptide produced by action of proteases on the ColE1 DNA relaxation complex. This finding suggests that the relaxation protein is bound to ColE1 DNA in the relaxation complex via a phosphodiester linkage between a serine hydroxyl of the protein and the 5'-phosphate of the terminal deoxycytidine residue of the DNA. |
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