Fibrinolytic components in nasal mucosa and nasal secretion |
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Authors: | Toyotoshi Yasuda Y Sakata Seiji Madoiwa Jun Mimuro Michio Matsuda Ken Kitamura |
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Institution: | (1) Department of Otorhinolaryngology, Jichi Medical School, Minamikawachi-machi, Tochigi 329–04, Japan, JP;(2) Division of Thrombosis and Hemostasis, Jichi Medical School, Minamikawachi-machi, Kawachi-gun, Tochigi 329–04, Japan e-mail:yoisaka@jichi.ac.jp Tel. +81-285-44-2111, ext 3337; Fax +81-285-44-7817, JP |
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Abstract: | We evaluated a possible role for fibrinolytic components in nasal secretion by tissue localization with immunohistochemical
techniques and by measuring their antigen concentrations in nasal discharge by means of ELISA and fibrin autography. Nasal
mucosa was obtained surgically from the inferior turbinate. Urokinase-type plasminogen activator (u-PA) specific staining
was observed in pseudostratified ciliated epithelium and was predominant in mucous cells of the seromucinous gland, while
serous cells were almost devoid of stain. The pattern of staining of plasminogen activator inhibitor-2 was similar to that
of u-PA. In contrast, plasminogen activator inhibitor-1(PAI-1) immunoreactive material was localized exclusively in serous
cells of seromucinous glands. Positive staining for tissue-type plasminogen activator (t-PA) was observed in endothelial cells
and basal cells, which differentiate into either ciliated or goblet cells. Nasal secretions were partially fractionated by
immunospecific antibody-immobilized Sepharose. Subsequent fibrin autography patterns indicated the presence of u-PA, PAI-1,
and t-PA. After methacholine provocation, the level of t-PA increased transiently but decreased rapidly with subsequent challenges.
These differential stainings of fibrinolytic components and the existence of PAs and PAI-1 in the nasal discharge suggest
that the fibrinolytic system may play a role in the movement and fluidity of nasal secretion.
Accepted: 25 May 1998 |
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