LPS,Oleuropein and Blueberry extracts affect the survival,morphology and Phosphoinositide signalling in stimulated human endothelial cells |
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Authors: | Vincenza Rita Lo Vasco Martina Leopizzi Valeria Di Maio Tania Di Raimo Stefania Cesa Alessandra Masci Carlo Della Rocca |
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Institution: | 1.Department of Sensory Organs, Faculty of Medicine and Dentistry, Policlinico Umberto I,Sapienza University of Rome,Rome,Italy;2.Department of Medico-Surgical Sciences and Biotechnology, Polo Pontino,Sapienza University,Rome,Italy;3.Medical Oncology Unit,San Filippo Neri Hospital,Rome,Italy;4.Department of Chemistry and Technology of Drugs,Sapienza University of Rome,Rome,Italy;5.Department of Experimental Medicine, Research Unit on Food Science and Human Nutrition,Sapienza University of Rome,Rome,Italy |
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Abstract: | Endothelial cells (EC) act as leading actors in angiogenesis. Understanding the complex network of signal transduction pathways which regulate angiogenesis might offer insights in the regulation of normal and pathological events, including tumours, vascular, inflammatory and immune diseases. The effects of olive oil and of Blueberry extracts upon the phosphoinositide (PI)-specific phospholipase C (PLC) enzymes were evaluated both in quiescent and inflammatory stimulated human umbilical vein EC (HUVEC) using molecular biology (multiliquid bioanalysis) and immunofluorescence techniques. Oleuropein significantly increased the number of surviving HUVEC compared to untreated controls, suggesting that it favours the survival and proliferation of EC. Our results suggest that Oleuropein might be useful to induce EC proliferation, an important event during angiogenesis, with special regard to wound healing. Blueberry extracts increased the number of surviving HUVEC, although the comparison to untreated controls did not result statistically significant. Lipopolysaccharide (LPS) administration significantly reduced the number of live HUVEC. LPS can also modify the expression of selected PLC genes. Adding Blueberry extracts to LPS treated HUVEC cultures did not significantly modify the variations of PLC expression induced by LPS. Oleuropein increased or reduced the expression of PLC genes, and statistically significant results were identified for selected PLC isoforms. Oleuropein also modified the effects of LPS upon PLC genes’ expression. Thus, our results corroborate the hypothesis that Oleuropein owns anti-inflammatory activity. The intracellular localization of PLC enzymes was modified by the different treatments we used. Podosome-like structures were observed in differently LPS treated HUVEC. |
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