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Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes |
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| Authors: | Mohammad Abu Hena Mostofa Jamal Satya Priya Sharma Hea-Jong Chung Hyeon-Jin Kim Seong-Tshool Hong Seungkoo Lee |
| Affiliation: | 1.Department of Biomedical Sciences and Institute for Medical Science, Chonbuk National University Medical School,Chonbuk National University,Jeonju,South Korea;2.JINIS BDRD Institute,JINIS Biopharmaceuticals Co.,Wanju,Korea;3.Department of Anatomic Pathology, School of Medicine, Kangwon National University Hospital,Kanwong National University,Chuncheon,South Korea;4.Department of Biotechnology and Genetic Engineering,Islamic University,Kushtia,Bangladesh;5.Department of Microbiology,Seonam University Medical School,Namwon,South Korea |
| Abstract: | Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to E. coli host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction. |
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