Genetic Diversity of <Emphasis Type="Italic">Arcobacter</Emphasis> Species of Animal and Human Origin in Andhra Pradesh,India |
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Authors: | M Soma Sekhar T Srinivasa Rao B K Chinnam K V Subramanyam M Metta N Mohammad Sharif |
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Institution: | 1.Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science,Sri Venkateswara Veterinary University,Gannavaram, Krishna (District),India;2.Department of Veterinary Microbiology,NTR College of Veterinary Science,Gannavaram,India;3.Department of AGB,NTR College of Veterinary Science,Gannavaram,India;4.Department of Veterinary Microbiology,College of Veterinary Science,Tirupati,India |
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Abstract: | Arcobacter is an emerging foodborne pathogen having zoonotic significance. Enterobacterial repetitive intergenic consensus (ERIC) PCR and repetitive sequence-based PCR (rep-PCR) analysis of a total of 41 Arcobacter isolates revealed a greater degree of genetic diversity. ERIC-PCR genotyping distinguished 14, 13 and 12 genotypes among 16, 13 and 12 isolates of A. butzleri, A. cryaerophilus and A. skirrowii, respectively. Rep-PCR genotyping distinguished 15, 12 and 11 genotypes among 16, 13 and 12 isolates of A. butzleri, A. cryaerophilus and A. skirrowii, respectively. The discriminatory power for ERIC and rep-PCR was found to be 0.997 and 0.996, respectively. Close clustering between isolates of animal and human origin are indicative of probable zoonotic significance. |
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