Functions of Conserved Motifs in the RNA-Dependent RNA Polymerase of a Yeast Double-Stranded RNA Virus |
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| Authors: | Eric Routhier and Jeremy A Bruenn |
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| Institution: | Department of Biological Sciences, SUNY—Buffalo, Buffalo, New York 14260 |
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| Abstract: | At least eight conserved motifs are visible in the totivirus RNA-dependent RNA polymerase (RDRP). We have systematically altered each of these in the Saccharomyces cerevisiae double-stranded RNA virus ScVL1 by substituting the conserved motifs from a giardiavirus. The results help define the conserved regions of the RDRP involved in polymerase function and those essential for other reasons. |
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