Purification, and properties of a bovine uricase |
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Authors: | Rajoka Muhammad Ibrahim Rehman Khalil-Ur- Mehraj Munazza Akhtar Muhammad Waheed Zia Muhammad Anjum |
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Affiliation: | National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan. mirajoka@nibge.org |
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Abstract: | Uricase from bovine kidney, purified to homogeneity level, had a molecular weight of 70 kDa. The apparent K(m) and V(max) values for uric acid hydrolysis were 0.125 mM and 102 IU mg(-1) protein respectively. The activation energy requirement for uric acid hydrolysis by uricase and inactivation of enzyme were 11.6 and 14.5 kJ/M respectively. Both enthalpy (Delta H*) and entropy of activation (Delta S*) for uricase activity were lower than those reported for some thermostable enzymes. |
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