Role of various growth media on shinorine (mycosporine-like amino acid) concentration and photosynthetic yield in <Emphasis Type="Italic">Anabaena variabilis</Emphasis> PCC 7937 |
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Authors: | Shailendra P Singh Manfred Klisch Donat-P Häder Rajeshwar P Sinha |
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Institution: | (1) Department of Plant Ecophysiology, University of Erlangen-Nuremberg, Staudtstrasse 5, 91058 Erlangen, Germany;(2) Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005, India |
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Abstract: | The induction of shinorine (mycosporine-like amino acid (MAA)) in Anabaena variabilis PCC 7937 was studied in various culture media (BGA−, BGA+, BG11 and Allen and Arnon’s). The objective was to select the most appropriate medium that can support the highest induction
of MAAs and can be used for industrial production of these UV protective substances from cyanobacteria. Also, in vivo photosynthetic
activity was measured under shinorine inducing conditions in all media. The shinorine content and photosynthetic activity
(yield) were highest (2948.73 ± 61.13 nmol/g dry wt and 0.47 ± 0.01, respectively) in BG11 medium in comparison to others
after 72 h of UV radiation. After the same duration of irradiation shinorine content was 1076.08 ± 21.77, 1320.07 ± 98.19
and 554.64 ± 16.47 nmol/g dry wt in BGA−, BGA+, and Allen and Arnon’s media, respectively. Thus, BG11 medium can be used for mass production of MAAs from cyanobacteria. |
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Keywords: | High performance liquid chromatography (HPLC) Mycosporine-like amino acid (MAA) Pulse amplitude modulated (PAM) fluorometer Sunscreen UV radiation |
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